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RodZ (YfgA) is required for proper assembly of the MreB actin cytoskeleton and cell shape in E. coli
Author(s) -
Bendezú Felipe O,
Hale Cynthia A,
Bernhardt Thomas G,
de Boer Piet A J
Publication year - 2009
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2008.264
Subject(s) - mreb , biology , cytoskeleton , microbiology and biotechnology , actin , cytoplasm , cell membrane , actin cytoskeleton , mutant , transmembrane protein , cell , genetics , gene , receptor
The bacterial MreB actin cytoskeleton is required for cell shape maintenance in most non‐spherical organisms. In rod‐shaped cells such as Escherichia coli , it typically assembles along the long axis in a spiral‐like configuration just underneath the cytoplasmic membrane. How this configuration is controlled and how it helps dictate cell shape is unclear. In a new genetic screen for cell shape mutants, we identified RodZ (YfgA) as an important transmembrane component of the cytoskeleton. Loss of RodZ leads to misassembly of MreB into non‐spiral structures, and a consequent loss of cell shape. A juxta‐membrane domain of RodZ is essential to maintain rod shape, whereas other domains on either side of the membrane have critical, but partially redundant, functions. Though one of these domains resembles a DNA‐binding motif, our evidence indicates that it is primarily responsible for association of RodZ with the cytoskeleton.