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Determination of bacterial rod shape by a novel cytoskeletal membrane protein
Author(s) -
Shiomi Daisuke,
Sakai Masako,
Niki Hironori
Publication year - 2008
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2008.234
Subject(s) - mreb , periplasmic space , biology , peptidoglycan , cytoplasm , cytoskeleton , microbiology and biotechnology , mutant , actin , morphogenesis , cell membrane , cell , escherichia coli , gene , biochemistry
Cell shape is critical for growth, and some genes are involved in bacterial cell morphogenesis. Here, we report a novel gene, rodZ , required for the determination of rod shape in Escherichia coli . Cells lacking rodZ no longer had rod shape but rather were round or oval. These round cells were smaller than known round mutant cells, including mreB and pbpA mutants; both are known to lose rod shape. Morphogenesis from rod cells to round cells and vice versa , caused by depletion and overproduction of RodZ, respectively, revealed that RodZ could regulate the length of the long axis of the cell. RodZ is a membrane protein with bitopic topology such that the N‐terminal region including a helix‐turn‐helix motif is in the cytoplasm, whereas the C‐terminal region is exposed in the periplasm. GFP–RodZ forms spirals along the lateral axis of the cell beneath the cell membrane, similar to the MreB bacterial actin. Thus, RodZ may mediate spatial information from cytoskeletal proteins in the cytoplasm to a peptidoglycan synthesis machinery in the periplasm.