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Localization of Smc5/6 to centromeres and telomeres requires heterochromatin and SUMO, respectively
Author(s) -
Pebernard Stephanie,
Schaffer Lana,
Campbell Daniel,
Head Steven R,
Boddy Michael N
Publication year - 2008
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2008.220
Subject(s) - biology , heterochromatin , centromere , telomere , genetics , heterochromatin protein 1 , microbiology and biotechnology , chromosome , dna , gene
The Smc5/6 holocomplex executes key functions in genome maintenance that include ensuring the faithful segregation of chromosomes at mitosis and facilitating critical DNA repair pathways. Smc5/6 is essential for viability and therefore, dissecting its chromosome segregation and DNA repair roles has been challenging. We have identified distinct epigenetic and post‐translational modifications that delineate roles for fission yeast Smc5/6 in centromere function, versus replication fork‐associated DNA repair. We monitored Smc5/6 subnuclear and genomic localization in response to different replicative stresses, using fluorescence microscopy and chromatin immunoprecipitation (ChIP)‐on‐chip methods. Following hydroxyurea treatment, and during an unperturbed S phase, Smc5/6 is transiently enriched at the heterochromatic outer repeats of centromeres in an H3‐K9 methylation‐dependent manner. In contrast, methyl methanesulphonate treatment induces the accumulation of Smc5/6 at subtelomeres, in an Nse2 SUMO ligase‐dependent, but H3‐K9 methylation‐independent manner. Finally, we determine that Smc5/6 loads at all genomic tDNAs, a phenomenon that requires intact consensus TFIIIC‐binding sites in the tDNAs.