Premium
LIS1 and NDEL1 coordinate the plus‐end‐directed transport of cytoplasmic dynein
Author(s) -
Yamada Masami,
Toba Shiori,
Yoshida Yuko,
Haratani Koji,
Mori Daisuke,
Yano Yoshihisa,
MimoriKiyosue Yuko,
Nakamura Takeshi,
Itoh Kyoko,
Fushiki Shinji,
Setou Mitsutoshi,
WynshawBoris Anthony,
Torisawa Takayuki,
Toyoshima Yoko Y,
Hirotsune Shinji
Publication year - 2008
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2008.182
Subject(s) - biology , dynein , cytoplasm , microbiology and biotechnology , dynein atpase , microtubule
LIS1 was first identified as a gene mutated in human classical lissencephaly sequence. LIS1 is required for dynein activity, but the underlying mechanism is poorly understood. Here, we demonstrate that LIS1 suppresses the motility of cytoplasmic dynein on microtubules (MTs), whereas NDEL1 releases the blocking effect of LIS1 on cytoplasmic dynein. We demonstrate that LIS1, cytoplasmic dynein and MT fragments co‐migrate anterogradely. When LIS1 function was suppressed by a blocking antibody, anterograde movement of cytoplasmic dynein was severely impaired. Immunoprecipitation assay indicated that cytoplasmic dynein forms a complex with LIS1, tubulins and kinesin‐1. In contrast, immunoabsorption of LIS1 resulted in disappearance of co‐precipitated tubulins and kinesin. Thus, we propose a novel model of the regulation of cytoplasmic dynein by LIS1, in which LIS1 mediates anterograde transport of cytoplasmic dynein to the plus end of cytoskeletal MTs as a dynein‐LIS1 complex on transportable MTs, which is a possibility supported by our data.