Open AccessDifferentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel
Author(s) -
OhradanovaRepic Anna,
Machacek Christian,
Fischer Michael B,
Stockinger Hannes
Publication year - 2016
Publication title -
clinical and translational immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.321
H-Index - 34
ISSN - 2050-0068
DOI - 10.1038/cti.2015.39
Subject(s) - cd14 , monocyte , monoclonal antibody , mononuclear phagocyte system , macrophage , immunology , biology , flow cytometry , immune system , peripheral blood mononuclear cell , dendritic cell , phagocyte , antibody , microbiology and biotechnology , in vitro , biochemistry
The mononuclear phagocyte system, consisting of monocytes, macrophages and dendritic cells (DCs), has an important role in tissue homeostasis as well as in eliciting immune responses against invading pathogens. Blood monocytes have been viewed for decades as precursors of tissue macrophages. Although the newest data show that in the steady state resident macrophages of many organs are monocyte independent, blood monocytes critically contribute to tissue macrophage and DC pools upon inflammation. To better understand the relationship between these populations and their phenotype, we isolated and differentiated human blood CD14 + monocytes in vitro into immature and mature monocyte‐derived dendritic cells (MoDCs) as well as into seven different monocyte‐derived macrophage subsets. We used the panel of 70 monoclonal antibodies (mAbs) submitted to the 10th Human Leukocyte Differentiation Antigen Workshop to determine the expression profiles of these 10 populations by flow cytometry. We now can compile subpanels of mAbs to differentiate the 10 monocyte/macrophage/MoDC subsets, providing the basis for novel diagnostic and therapeutic tools.