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Intracellular Drug Concentrations
Author(s) -
Dollery C T
Publication year - 2013
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1038/clpt.2012.240
Subject(s) - intracellular , cytoplasm , chemistry , drug , mass spectrometry , organelle , transporter , biophysics , clinical pharmacology , mass spectrometry imaging , computational biology , pharmacology , biochemistry , chromatography , biology , gene
Many drug targets are intracellular. To access them, a drug molecule must pass through the cell membrane, a process often facilitated or impeded by transporters. Once in the cytoplasm, basic molecules may become concentrated in organelles. To predict the pharmacologic effect accurately, there must be data concerning the concentration at the target, which is difficult to measure. Techniques that combine mass spectrometry and imaging techniques (matrix‐assisted laser desorption/ionization, secondary ion mass spectrometry (SIMS), and nanoSIMS) have promise in addressing this problem. Clinical Pharmacology & Therapeutics (2013); 93 3, 263–266. doi: 10.1038/clpt.2012.240