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Prolonged Use of Aspirin Alters Human and Rat Intestinal Cells and Thereby Limits the Absorption of Clopidogrel
Author(s) -
Jung KH,
Chu K,
Lee ST,
Yoon HJ,
Chang JY,
Nam WS,
Yoon SH,
Cho JY,
Yu KS,
Jang IJ,
Kim M,
Lee S K,
Roh JK
Publication year - 2011
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1038/clpt.2011.163
Subject(s) - aspirin , clopidogrel , pharmacology , in vivo , medicine , platelet , absorption (acoustics) , downregulation and upregulation , caco 2 , efflux , small intestine , chemistry , in vitro , biology , biochemistry , gene , materials science , microbiology and biotechnology , composite material
Clopidogrel therapy to prevent atherothrombosis faces the challenge of reduced responsiveness. The absorption of clopidogrel is regulated by multidrug‐resistance protein 1 (MDR1) in the intestinal epithelium. Given that aspirin induces MDR1 in cancer cells and peripheral blood cells, it may induce MDR1 in intestinal epithelial cells as well, thereby affecting the absorption of clopidogrel. In this study, aspirin treatment induced the expression of MDR1 in human epithelial colorectal (Caco‐2) cells in vitro and in rat intestine in vivo , as evidenced by dose‐dependent increases in gene, protein, and efflux function. Along with the upregulation of MDR1 proteins by aspirin, clopidogrel absorption was significantly decreased in the aspirin‐treated Caco‐2 cells and in rat intestine. Our data provide evidence that prolonged use of aspirin may reduce the intestinal absorption of clopidogrel. Further human studies would be necessary to clarify whether these data have any relevance to prevention of stroke or myocardial infarction. Clinical Pharmacology & Therapeutics (2011) 90 4, 612–619. doi: 10.1038/clpt.2011.163