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Analysis of the CYP2D6 gene in relation to debrisoquin and desipramine hydroxylation in a Swedish population
Author(s) -
Dahl MarjaLiisa,
Johansson Inger,
Palmertz Margareta Porsmyr,
IngelmanSundberg Magnus,
Sjöqvist Folke
Publication year - 1992
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1038/clpt.1992.2
Subject(s) - restriction fragment length polymorphism , debrisoquine , biology , cyp2d6 , allele , genetics , microbiology and biotechnology , desipramine , population , gene , polymerase chain reaction , genotype , medicine , endocrinology , environmental health , antidepressant , hippocampus
The molecular basis of polymorphic debrisoquin hydroxylation was studied in 223 Swedish white subjects, 187 extensive metabolizers and 36 poor metabolizers phenotyped with debrisoquin and desipramine. Restriction fragment length polymorphism (RFLP) analysis of the CYP2D6 gene revealed that 52% of unrelated poor metabolizers were homozygous for Xba I 29 kb fragment, and only 8% had two mutant alleles detected with RFLP. Alkie‐specific polymerase chain reaction (PCR)?based DNA amplification, however, revealed that all but one of the poor metabolizers had two mutant alleles of the CYP2D6A or CYP2D6B type or both. Extensive metabolizers who were heterozygous for wild‐type and CYP2D6B genes had metabolic ratios for debrisoquin and desipramine that were higher than those of subjects who were homozygous for the wild‐type gene. The 16 + 9 kb Xba I RFLP pattern was associated with the poor metabolizer phenotype and CYP2D6B mutations. Three extremely rapid metabolizers of debrisoquin had a 44 kb Xba I fragment that did not carry either CYP2D6A or CYP2D6B mutations. In conclusion, in the Swedish population studied, allele‐specific PCR amplification allowed prediction of the debrisoquin hydroxylation phenotype with 99% accuracy. Clinical Pharmacology and Therapeutics (1992) 51 , 12–17; doi: 10.1038/clpt.1992.2