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Digoxigenin biotransformation
Author(s) -
Gault Henry,
Kalra Jawahar,
Longerich Linda,
Dawe Madonna
Publication year - 1982
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1038/clpt.1982.98
Subject(s) - digoxigenin , digoxin , biotransformation , chemistry , chromatography , metabolite , radioimmunoassay , high performance liquid chromatography , pharmacology , biochemistry , medicine , enzyme , in situ hybridization , heart failure , gene expression , gene
Two healthy subjects took 3 H‐digoxigenin‐12α and unlabeled digoxigenin. Metabolites were assayed by high‐pressure liquid chromatography (HPLC) in serum and urine. Of the tritium activity in the serum at 30 min, less than 26% chromatographed with digoxigenin; the rest chromatographed as metabolites, most of which were polar. The main polar metabolites identified were glucuronides of 3‐epidigoxigenin. An important route of biotransformation to polar metabolites appears to be from 3β‐digoxigenin through 3‐keto‐digoxigenin to 3‐epidigoxigenin. Several HPLC peaks remain unidentified. There was extensive cross reactivity between metabolites and antisera to digoxin. The digoxigenin route of digoxin biotransformation to polar metabolites may be important in some patients receiving digoxin and such metabolites could contribute an important fraction to the serum digoxin concentration measured by radioimmunoassay. Clinical Pharmacology and Therapeutics (1982) 31 , 695–704; doi: 10.1038/clpt.1982.98