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Smoking‐induced changes in nicotine disposition: Application of a new HPLC assay for nicotine and its metabolites
Author(s) -
Kyerematen Gabriel A,
Damiano Michael D,
Dvorchik Barry H,
Vesell Elliot S
Publication year - 1982
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1038/clpt.1982.235
Subject(s) - nicotine , cotinine , pharmacology , chemistry , urine , volume of distribution , elimination rate constant , morning , disposition , clinical pharmacology , high performance liquid chromatography , absorption (acoustics) , chromatography , pharmacokinetics , medicine , biochemistry , social psychology , physics , psychology , acoustics
A sensitive, rapid high‐pressure liquid chromatographic assay was developed to compare the disposition of an intravenous dose of l4 C‐nicotine in normal, carefully matched smokers and nonsmokers. The elimination half‐lifes of nicotine and cotinine were shorter in smokers than in nonsmokers. Also consistent with an inductive effect of smoking was the increased nicotine elimination rate constant in smokers, but smoking induced more complex kinetic changes: nicotine volume of distribution was diminished in smokers, whereas nicotine clearance and area under the concentration‐time curve were unchanged. The presence of nicotine and its principal metabolites in a morning specimen of urine obtained from nonsmokers before 14 C‐nicotine administration suggests ubiquitous, passive exposure to and absorption of chemicals present in cigarette smoke. Clinical Pharmacology and Therapeutics (1982) 32 , 769–780; doi: 10.1038/clpt.1982.235