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Absence of seasonal variation in antipyrine metabolism
Author(s) -
Paigen Beverly,
Ward Elizabeth,
Steenland Kyle,
Bolanowska Wanda,
Gessner Teresa,
Chang Richard L,
Wood Alexander W,
Conney Allan H
Publication year - 1982
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1038/clpt.1982.23
Subject(s) - metabolism , in vivo , chemistry , microsome , unspecific monooxygenase , monooxygenase , drug metabolism , pyrene , in vitro , cytochrome , benzanthracene , cytochrome p450 , medicine , endocrinology , biochemistry , carcinogen , biology , enzyme , microbiology and biotechnology , organic chemistry
The induced activity of aryl hydrocarbon hydroxylase (AHH), measured by the metabolism of benzo[ a ]pyrene to fluorescent products in cultured human lymphocytes, shows a strong seasonal variation. The in vivo metabolism of antipyrine, which is also catalyzed by microsomal cytochrome P‐450‐dependent monooxygenases, has been reported to be correlated with AHH inducibility in human lymphocytes. To determine whether antipyrine metabolism also showed seasonal changes, we measured antipyrine half‐life (t½) in 10 nonsmokers and eight smokers at the two times of the year that correspond to the high and low peaks of inducible AHH activity as measured in lymphocytes. The mean antipyrine t½ determined in all 18 subjects in summer was almost identical to that found in winter ( x ± SEM = 10.90 ± 0.65 and 10.96 ± 0.78 hr). AHH activity in cultured human lymphocytes from the nonsmoking subjects was determined in control and 3‐methylcholanthrene–induced cells to obtain inducibility ratios of 4.2 ± 0.56 (SEM) in the summer and 1.4 ± 0.14 (SEM) in the winter. These results indicate that the seasonal variation in AHH inducibility in human lymphocytes is not reflected by a corresponding seasonal variation in antipyrine metabolism in vivo. Clinical Pharmacology and Therapeutics (1982) 31 , 144–150; doi: 10.1038/clpt.1982.23