Premium
The antiarrhythmic effect of centrally administered rilmenidine involves muscarinic receptors, protein kinase C and mitochondrial signalling pathways
Author(s) -
Iwasaki M,
Hayashi Y,
Kamibayashi T,
Yamatodani A,
Mashimo T
Publication year - 2008
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/bjp.2008.35
Subject(s) - rilmenidine , imidazoline receptor , pharmacology , muscarinic acetylcholine receptor , mitochondrial permeability transition pore , chemistry , endocrinology , halothane , medicine , agonist , receptor , biology , biochemistry , apoptosis , programmed cell death , organic chemistry
Background and purpose: We have previously demonstrated that stimulation of imidazoline receptors in the CNS prevented halothane–adrenaline arrhythmias during halothane anaesthesia and that stimulation of the vagus nerve may be critical to this effect. However, details of the mechanism(s) involved are not yet available. The present study was designed to examine the role of muscarinic receptors, protein kinase C (PKC), ATP‐sensitive potassium channels (K ATP ) and the mitochondrial permeability transition pore (MPTP) in the antiarrhythmic effect of rilmenidine, an imidazoline receptor agonist. Experimental approach: Rats were anaesthetized with halothane and monitored continuously for arterial blood pressure and premature ventricular contractions. The arrhythmogenic dose of adrenaline was defined as the lowest dose producing three or more premature ventricular contractions within a 15‐s period. We confirmed that centrally administered rilmenidine prevented halothane–adrenaline arrhythmias and then examined the antiarrhythmic effect of rilmenidine in the presence of atropine methylnitrate, a muscarinic receptor antagonist, calphostin C, a PKC inhibitor, HMR‐1098, a sarcolemmal K ATP inhibitor, 5‐hydroxydecanoic acid, a mitochondrial K ATP inhibitor or atractyloside, an MPTP opener. Key results: The antiarrhythmic effect of rilmenidine was significantly inhibited by atropine methylnitrate, calphostin C, 5‐hydroxydecanoic acid and atractyloside, but the effects of HMR‐1098 in our model were not clear. Conclusions and implications: The present results suggest that muscarinic receptors, PKC, mitochondrial K ATP channels and MPTP may be crucial components of the mechanism involved in the antiarrhythmic effect of rilmenidine given into the CNS. British Journal of Pharmacology (2008) 153 , 1623–1630; doi: 10.1038/bjp.2008.35 ; published online 25 February 2008