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trans ‐Resveratrol, an extract of red wine, inhibits human eosinophil activation and degranulation
Author(s) -
Tan Y,
Lim L H K
Publication year - 2008
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1038/bjp.2008.330
Subject(s) - resveratrol , eosinophil , chemistry , degranulation , eosinophil peroxidase , biochemistry , pharmacology , biology , immunology , enzyme , peroxidase , receptor , asthma
Background and purpose: t rans ‐Resveratrol, a non‐flavonoid polyphenol found abundantly in red wine possesses antiproliferative and anti‐inflammatory activity in various inflammatory disease conditions. However, the effect of trans ‐resveratrol on eosinophil activation in relation to allergy has not been investigated. Experimental approach: Human eosinophils were isolated and purified from whole blood and incubated for 16 h with trans ‐resveratrol. Eosinophil chemotaxis, activation and degranulation, and apoptosis were investigated. The effect of trans ‐resveratrol on the inhibition of p38 and ERK1/2 activation was examined. Key results: Treatment of human eosinophils with trans ‐resveratrol at concentrations <100 μ M for 16 h did not induce eosinophil apoptosis. Similar results were seen after 24 h and 48 h incubations. trans ‐Resveratrol (<100 μ M ) significantly inhibited eosinophil peroxidase release after activation with IL‐5 (IC 50 =2.9±0.9 μ M ) or C5a (IC 50 =3.9±0.5 μ M ) after 5 min priming with cytochalasin B (CB). Similarly, the production of leukotriene C4 after stimulation with calcium ionophore, and eosinophil chemotaxis in response to eotaxin, as well as CD11b upregulation and CD62 L shedding was also significantly reduced by trans ‐resveratrol, at concentrations above 5 μ M . All the activators induced p38 and ERK1/2 phosphorylation maximal at 2 min of activation. trans ‐Resveratrol potently inhibited p38 and ERK1/2 activation after calcium ionophore and CB and C5a activation. Conclusions and implications: trans ‐Resveratrol is effective at inhibiting human eosinophil activation and degranulation at concentrations <100 μ M , while not inducing apoptosis. This potent anti‐inflammatory activity of trans ‐resveratrol and possibly its metabolites on eosinophils may be worth investigating for the treatment of eosinophil‐related allergic diseases. British Journal of Pharmacology (2008) 155 , 995–1004; doi: 10.1038/bjp.2008.330 ; published online 8 September 2008