Open AccessHIV-1 Nef mediates lymphocyte chemotaxis and activation by infected macrophages
Author(s) -
Simon Swingler,
Angela M. Mann,
Jean Marc Jacqué,
Beda Břicháček,
Vito G. Sasseville,
Ken Williams,
Andrew A. Lackner,
Edward N. Janoff,
R. Wang,
Daniel Fisher,
Mario Stevenson
Publication year - 1999
Publication title -
nature medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.536
H-Index - 547
eISSN - 1546-170X
pISSN - 1078-8956
DOI - 10.1038/12433
Subject(s) - chemokine , chemotaxis , macrophage , biology , virology , viral replication , macrophage inflammatory protein , virus , lentivirus , microbiology and biotechnology , immunology , inflammation , in vitro , viral disease , genetics , receptor
Infection of macrophage lineage cells is a feature of primate lentivirus replication, and several properties of primate lentiviruses seem to have evolved to promote the infection of macrophages. Here we demonstrate that the accessory gene product Nef induces the production of two CC-chemokines, macrophage inflammatory proteins 1alpha and 1beta, by HIV-1-infected macrophages. Adenovirus-mediated expression of Nef in primary macrophages was sufficient for chemokine induction. Supernatants from Nef-expressing macrophages induced both the chemotaxis and activation of resting T lymphocytes, permitting productive HIV-1 infection. These results indicate a role for Nef in lymphocyte recruitment and activation at sites of virus replication.