Open AccessKaryotypic characterization of representatives from Melolonthinae (Coleoptera: Scarabaeidae): karyotypic analysis, banding and fluorescent in situ hybridization (FISH)
Author(s) -
DE CÁSSIA DE MOURA RITA,
DE SOUZA MARIA JOSÉ,
DE MELO NATONIEL FRANKLIN,
DE CASTRO LIRANETO AMARO
Publication year - 2003
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1034/j.1601-5223.2003.01611.x
Subject(s) - biology , bivalent (engine) , ploidy , nucleolus organizer region , genetics , zoology , chromosome , gene , metal , chemistry , organic chemistry
Meiotic chromosomes of Phyllophaga ( Phytalus ) vestita , Phyllophaga ( Phyllophaga ) aff capillata and Lyogenys fuscus (Melolonthinae) were analyzed by conventional staining, C‐banding, fluorochromes, silver nitrate and FISH. The three species had a diploid number of 2n=20 and a sex mechanism of the (Xy p ; XY p ) parachute type. P. ( Phytalus ) vestita, P. ( Phyllophaga ) aff capillata and Lyogenys fuscus showed pericentromeric constitutive heterochromatin (CH) in all autosomal bivalents and on X chromosomes. Staining with CMA 3 and DAPI fluorochromes showed that the CH of P. ( Phytalus ) vestita is not specifically rich in AT and GC‐base pairs, whereas in P. ( Phyllophaga ) aff capillata the sex bivalent and one autosomal pair were found to be enriched in GC base pairs with CMA 3 , and in Lyogenys fuscus CH was positive for DAPI. Silver nitrate staining revealed nucleolar remnants in all three species. However, FISH obtained a precise identification of nucleolar organizing regions with an rDNA 18S and 25S probe. A signal of hybridization was seen in each species, being detected in the X chromosome of P. ( Phytalus ) vestita and Lyogenys fuscus , and in a small autosomal bivalent of P. ( Phyllophaga ) aff capillata .