Open AccessRepeated DNA sequences in the microbat species Miniopterus schreibersi (Vespertilionidae; Chiroptera)
Author(s) -
BARRAGÁN M. J. L.,
MARTÍNEZ S.,
MARCHAL J. A.,
BULLEJOS M.,
DÍAZ DE LA GUARDIA R.,
SÁNCHEZ A.
Publication year - 2002
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1034/j.1601-5223.2002.1370109.x
Subject(s) - biology , repeated sequence , genome , hpaii , genetics , ecori , tandem repeat , interspersed repeat , dna , dna sequencing , dna methylation , southern blot , evolutionary biology , restriction enzyme , gene , human genome , gene expression
Repetitive DNA sequences represent a substantial component of eukaryotic genomes. These sequences have been described and characterized in many mammalian species. However, little information about repetitive DNA sequences is available in bat species. Here we describe an EcoR I family of repetitive DNA sequences present in the species Miniopterus schreibersi . These repetitive sequences are 57.85% A‐T rich, organized in tandem, and with a monomer unit length of 904 bp. Methylation analysis using the isoesquizomer pair Msp I and Hpa II indicates that the cytosines present in the sequences CCGG are partially methylated. Furthermore, Southern blot analysis demonstrated that these DNA sequences are absent in the genomes of four related microbat species and suggest that it could be specific to the M. schreibersi genome.