Open AccessOptimization of sorghum transformation parameters using genes for green fluorescent protein and β‐glucuronidase as visual markers
Author(s) -
JEOUNG J. M.,
KRISHNAVENI S.,
MUTHUKRISHNAN S.,
TRICK H. N.,
LIANG G. H.
Publication year - 2002
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1034/j.1601-5223.2002.1370104.x
Subject(s) - transformation (genetics) , green fluorescent protein , biology , beta glucuronidase , reporter gene , transgene , explant culture , agrobacterium , gene , glucuronidase , transformation efficiency , microbiology and biotechnology , gus reporter system , genetically modified crops , sorghum , gene expression , genetics , in vitro , ecology
Early and reliable detection of plant transformation events is essential for establishing efficient transformation protocols. We have compared the effectiveness of using the gene encoding a green fluorescent protein (GFP) and a β‐glucuronidase ( gus ) as reporter genes for early detection of transgene expression in explants subjected to biolistic bombardment and Agrobacterium ‐mediated transformation. The results indicate that gfp gene is superior to gus gene in following transgene expression in transiently transformed materials in both methods of transformation. Using GFP as the screenable marker, we have optimized sorghum transformation with respect to the conditions for transformation, type of explants, promoters, and inbreds. These optimized conditions have been used to obtain stably transformed explants for subsequent regeneration.