Open AccessISSR (Inter Simple Sequence Repeats) as genetic markers in Noctuids (Lepidoptera)
Author(s) -
LUQUE C.,
LEGAL L.,
STAUDTER H.,
GERS C.,
WINK M.
Publication year - 2002
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1034/j.1601-5223.2002.1360312.x
Subject(s) - biology , microsatellite , lepidoptera genitalia , primer (cosmetics) , genome , amplified fragment length polymorphism , genetics , genomic dna , genetic variation , evolutionary biology , genetic diversity , dna , botany , gene , population , allele , chemistry , demography , organic chemistry , sociology
Microsatellites among Lepidoptera are poorly known. We have shown that some ISSR amplifications are possible and demonstrate their applicability in studying intraand inter-specific variation in some Noctuid populations. The Lepidoptera are sensitive to habitat and climatic change (HARPER et al. 2000). We analyse Noctuid populations in natural and disturbed habitats in the Pyrenees (France). We have attempted to find a versatile method that can be used in species determination, and, to demonstrate small genetic differences, in the nuclear genome of moths. Compared to other groups of animals relatively few microsatellite studies have been carried out with Lepidoptera (NEVE and MEGLECZ 2000). Only few studies involve ISSR (REDDY et al. 1999). One reason was that it has been assumed that microsatellites are lacking or are very rare in Lepidoptera. However, MEGLECZ and SOLIGNAC (1998) and HARPER et al. (2000) have successfully localised (CA)n repeats in insect microsatellites. We found that: (i) (CA)n primer gives the most informative profiles; (ii) DNA profiles between species differ substantially; (iii) comparison of ISSR profiles can be successfully applied to study intra-specific variation.