Open AccessNew C‐band protocol by heat denaturation in the presence of formamide
Author(s) -
FERNÁNDEZ R.,
BARRAGÁN M. J. L.,
BULLEJOS M.,
MARCHAL J. A.,
DÍAZ DE LA GUARDIA R.,
SÁNCHEZ A.
Publication year - 2002
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1034/j.1601-5223.2002.01672.x
Subject(s) - formamide , denaturation (fissile materials) , biology , barium hydroxide , incubation , heterochromatin , dna , constitutive heterochromatin , microbiology and biotechnology , biochemistry , genetics , nuclear chemistry , chemistry , inorganic chemistry , chromatin
C‐banding techniques detect the presence of constitutive heterochromatin, which is usually located in centromeric regions of chromosomes in the majority of analysed species. The common method for C‐banding used over the last 30 years involves treatment with a mild alkali barium hydroxide 5 % Ba(OH) 2 at 50°C for 5–15 min and subsequent incubation in salt solution (2×SSC at 60°C for 1 h). We here present a new, easy and reliable technique for C‐banding, which basically involves heat denaturation of chromosomal DNA in the presence of formamide and incubation in 2×SSC at room temperature.