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Inhibitory effect of titanium particles on osteoclast formation generated by treatment of mouse bone marrow cells with PGE 2
Author(s) -
Nakano M,
Tsuboi T,
Kato M,
Kurita K,
Togari A
Publication year - 2003
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1034/j.1601-0825.2003.02904.x
Subject(s) - osteoclast , rankl , bone marrow , chemistry , prostaglandin e2 , activator (genetics) , bone resorption , in vitro , medicine , microbiology and biotechnology , receptor , endocrinology , biochemistry , biology
OBJECTIVE: The present study was designed to evaluate the effect of titanium (Ti) particles with no endotoxin on osteoclast differentiation and osteoclast activity in in vitro experiments. METHODS: Osteoclast formation as well as osteoclastic bone resorbing activity were examined using the mouse bone marrow culture system and purified rabbit osteoclasts treated with Ti particles (2.5–20 μ g cm −2 ). RESULTS: Ti particles, with no adherent endotoxin, inhibited osteoclastogenesis and receptor activator of NF‐ κ B ligand (RANKL) expression in bone marrow cells treated with prostaglandin E 2 (PGE 2 ) (100 nM). The inhibitory effect of Ti particles was concentration‐dependent (5–20 μ g cm −2 ), and was observed only on the generation of osteoclasts by PGE 2 , but not by 1,25‐dihydroxyvitamin D 3 or soluble RANKL. This suggests that Ti particles did not act uniformly on a common process in the generation of osteoclasts, but specifically on signal transduction for PGE 2 in generating osteoclasts. In highly purified osteoclasts, Ti particles showed no effect on survival and bone resorbing activity. CONCLUSION: Ti particles inhibited osteoclast differentiation and RANKL expression in mouse bone marrow cells treated with PGE 2 , without affecting mature osteoclast survival or activity. Thus, Ti particles may alter the osteoclastogenetic action of PGE 2 , which is one of the regulatory factors of bone remodeling.