Premium
Enhancement of matrix metalloproteinase (MMP)‐2 activity in gingival tissue and cultured fibroblasts from Down's syndrome patients
Author(s) -
Komatsu T,
Kubota E,
Sakai N
Publication year - 2001
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1034/j.1601-0825.2001.70110.x
Subject(s) - matrix metalloproteinase , pathogenesis , gingivitis , periodontitis , messenger rna , zymography , reverse transcription polymerase chain reaction , tissue inhibitor of metalloproteinase , microbiology and biotechnology , pathology , biology , chemistry , medicine , gene , biochemistry , dentistry
OBJECTIVES: To identify one of the possible factors responsible for periodontal disease in Down's syndrome (trisomy 21) patients, we studied the enzyme activity and the mRNA expression pattern of matrix metalloproteinases (MMPs) of cultured gingival fibroblasts (GF) and fresh gingival tissues. MATERIALS AND METHODS: Gingival tissue was used as the cell source and was biopsied at the time of dental treatment from nine patients with Down's syndrome and nine non‐Down's controls. GF were cultivated in serum‐free media for analyses of their MMP activities at the transcription or the protein level. The MMP activities in the supernates were measured by gelatin impregnated zymography. Relative levels of MMP mRNA from the cultured GF or freshly isolated gingival tissues were determined using the reverse transcription polymerase chain reaction (RT‐PCR). RESULT AND CONCLUSIONS: The production of the active type of MMP‐2 in GF from Down's syndrome patients (D‐GF) was found to be significantly higher ( P < 0.05) than that of the control GF (C‐GF) at the protein level. The mRNA expressions of membrane‐type1 MMP (MT1‐MMP) and MMP‐2 in D‐GF were constitutively augmented when compared with those of C‐GF. These findings suggest that specific increase of the active form of MMP‐2 in D‐GF may possibly be due to the concomitant expression of MT1‐MMP in the cultured cells, and this could be related to the pathogenesis of gingivitis/periodontitis associated with Down's syndrome patients.