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Improved Islet Yields From Macaca Nemestrina and Marginal Human Pancreata After Two‐Layer Method Preservation and Endogenous Trypsin Inhibition
Author(s) -
Matsumoto Shinichi,
Rigley Theodore H.,
Reems Jo Anna,
Kuroda Yoshikazu,
Stevens R. Brian
Publication year - 2003
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1034/j.1600-6143.2003.30110.x
Subject(s) - islet , transplantation , medicine , pancreas , biology , endocrinology , diabetes mellitus
We tested whether two‐layer method (TLM) pancreas preservation and trypsin inhibition (Pefabloc) during processing allows longer preservation while retaining or improving viable islet recovery. Non‐marginal primate ( Macaca nemestrina ) and marginal human (ischemic or preservation‐injured) pancreata were processed with a research‐oriented pan technique (Seattle method). Organs were processed upon arrival (± Pefabloc), or after TLM or University of Wisconsin solution (UW) preservation (+ Pefabloc). Islet yield, viability, and function were assessed . Pefabloc increased M. nemestrina islet yields from 9696 ± 1749 IE/g to 15 822 ± 1332 IE/g (p < 0.01). Two‐layer method preservation (< 6 h) further increased yields, to 23 769 ± 2773 IE/g (vs. + Pefabloc; p < 0.01). Similarly, Pefabloc increased marginal human islet yields from 2473 ± 472 IE/g to 4723 ± 1006 IE/g (p < 0.04). This increase was maintained after lengthy TLM preservation (> 30 h; 4801 ± 1066 IE/g) . We also tested the applicability of TLM preservation (23.5 ± 3.2 h) to the processing of marginal human pancreata by the Edmonton/Immune Tolerance Network clinical protocol. Islet yield and function approached published results of pancreata processed 4.8 ± 0.8 h after organ recovery (p = 0.06) . Pefabloc, and TLM vs. UW preservation, prolonged the tolerable interval between organ recovery and islet isolation. Islet yield, viability, and functionality improved from both marginal and nonmarginal pancreata .