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In Vivo Imaging of Acute Cardiac Rejection in Human Patients Using 99m Technetium Labeled Annexin V
Author(s) -
Kown Murray H.,
Strauss H. William,
Blankenberg Francis G.,
Berry Gerald J.,
StaffordCecil Sandy,
Tait Jonathan F.,
Goris Michael L.,
Robbins Robert C.
Publication year - 2001
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1034/j.1600-6143.2001.001003270.x
Subject(s) - medicine , annexin , endomyocardial biopsy , ventricle , phosphatidylserine , annexin a5 , pathology , apoptosis , biopsy , in vivo , radiology , staining , phospholipid , biochemistry , chemistry , membrane , microbiology and biotechnology , biology , genetics
Annexin V binds phosphatidylserine moieties on apoptotic cells. This study reports the initial experience at Stanford University Medical Center with 99m Tc‐labeled annexin V imaging as a noninvasive measure of apoptosis in acute cardiac rejection. Ten cardiac transplant patients had 99m Tc Annexin V imaging and endomyocardial biopsy (EMB) performed within 24 h. No complications related to 99m Tc annexin V administration occurred. Eight patients had ISHLT grade of acute rejection of 1A or less. Five patients had two or more areas of uptake noted in the right ventricle on imaging studies. Two of these patients had positive biopsies: one patient had grade 2 rejection with two focal uptake areas and another had grade 3A rejection with three foci. An additional five patients had either one or zero hot spot areas and corresponding negative EMBs. 99m Tc‐annexin V appears to be well tolerated and may identify patients with acute cardiac rejection.