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Anti‐annexin A5 Antibodies in Reproductive Failures in Relation to Antiphospholipid Antibodies and Phosphatidylserine
Author(s) -
Arai T.,
Matsubayashi H.,
Sugi T.,
Kondo A.,
Shida M.,
Suzuki T.,
Izumi S.I.,
McIntyre J. A.,
Makino T.
Publication year - 2003
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2003.00069.x
Subject(s) - antibody , annexin a5 , phosphatidylserine , in vitro fertilisation , annexin , epitope , antiphospholipid syndrome , immunology , abortion , antigen , pregnancy , trophoblast , medicine , andrology , fetus , biology , phospholipid , biochemistry , placenta , genetics , flow cytometry , membrane
Problem: The presence of IgG anti‐annexin A5 (IgGαA5) and/or antiphospholipid antibodies (aPL) are risk factors associated with recurrent spontaneous abortion. Problems are whether IgA anti‐annexin A5 (IgAαA5) is pathogenic, and how IgGαA5 works. Method of study: Blood samples from 238 patients with early recurrent spontaneous abortion, 48 patients with recurrent in vitro fertilization‐embryo transfer failure, 179 non‐pregnant women and 120 pregnant controls were tested for IgAαA5 by enzyme‐linked immunosorbent assay. We also determined if IgGαA5 appeared coincident with aPL. The antigenic epitope(s) recognized by IgGαA5 was investigated. Results: We observed no difference between patients and controls for IgAαA5. The prevalence of IgGαA5 was not different statistically between patient samples with or without aPL. Patient IgGαA5 bound annexin A5 when the latter was free/unbound but not when annexin A5 was associated with phospholipid. Conclusions: The IgAαA5 does not appear to be pathogenic. IgGαA5 works to make a complex with annexin A5 without relation to aPLs, which may reduce annexin A5 available for binding to trophoblast.