Premium
Possible Modulators of IL‐8 and GRO‐α Production by Granulosa Cells
Author(s) -
Zeineh Kosay,
Kawano Yasushi,
Fukuda Junichiro,
Nasu Kaei,
Narahara Hisashi,
Miyakawa Isao
Publication year - 2003
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2003.00057.x
Subject(s) - endocrinology , tumor necrosis factor alpha , medicine , ovulation , cytokine , interleukin , protein kinase c , cyclic adenosine monophosphate , alpha (finance) , biology , chemistry , microbiology and biotechnology , kinase , receptor , hormone , construct validity , nursing , patient satisfaction
Problem: Interleukin‐8 (IL‐8) and growth‐regulated oncogene‐ α (GRO‐ α ) have been proved to be important modulators of leukocyte chemotaxis in the mechanism of human ovulation. This study investigated the possible effects of IL‐1 α , tumor necrosis factor‐ α (TNF‐ α ), protein kinase C (PKC) activators (TPA), and db‐cyclic adenosine monophosphate (cAMP) on IL‐8 and GRO‐ α production by immortalized GC1a and granulosa‐lutein cells. Method of Study: Confluent granulosa‐lutein cells were placed in serum‐free medium before incubated for 8 hr with the above‐mentioned test agents. Finally, we measured IL‐8 and GRO‐ α levels in the culture media using an enzyme‐linked immunosorbent assay (ELISA). Results: Treatment of granulosa‐lutein cells with of IL‐1 α (1 nM), TNF‐ α (1 nM), TPA (1 nM) and db‐cAMP (100 μ M) produced higher levels of IL‐8 than untreated cells by 8 hr (2274.7 ± 146.3, 1489.8 ± 190.1, 1452.9 ± 152.7, 1313.6.6 ± 48.4 pg/mL, respectively; control = 457.7 ± 38.2 pg/mL; P < 0.001). Treatment of granulosa‐lutein cells with 1 nM of IL‐1 α , TNF α , TPA, and db‐cAMP (100 μM) resulted in higher levels of GRO‐ α than untreated cells by 8 hr (993.7 ± 9.5, 171.4 ± 6.5, 147.5 ± 6.7, 472.4 ± 16.2 pg/mL respectively; control = 73.8 ± 8.2 pg/mL; P < 0.001). Conclusions: Our data strongly suggests roles for IL‐1 α , TNF α , and PKC activators in the inflammation–like mechanism of human ovulation. Furthermore, our study suggests a positive, but still debatable, role for cAMP in the same mechanism.