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In vitro Regulation of β 1 and β 3 Integrin Subunits in Endometrial Epithelial Cells From Normal Endometrium
Author(s) -
Pierro Emilia,
Minici Francesca,
Alesiani Ornella,
Monica Loredana D.,
Anna Maria M,
Muncuso Salvatore,
Lanzone Antonio
Publication year - 2003
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2003.00040.x
Subject(s) - endometrium , in vitro , integrin , microbiology and biotechnology , chemistry , biology , medicine , endocrinology , receptor , biochemistry
OBJECTIVE: To evaluate the effect of prostaglandin E 2 (PGE 2 ) and interleukin‐1β (IL‐1β) on integrin expression. DESIGN: Cultures of endometrial epithelial cells from normal endometrium. SETTING: All endometrial specimens were obtained from the Obstetrics and Gynecology Department of the Catholic University, Rome, Italy. PATIENTS: Four patients with normal menstrual cycles undergoing operative laparoscopy for non‐endometrial problems. INTERVENTION: Endometrial samples were collected by uterine courettings. MAIN OUTCOME MEASURE: Immunocytochemistry for β1 and β3 integrin subunits. RESULTS: PGE 2 clearly enhances both β1 and β3 integrin subunit expression. IL‐1β seem to slightly increase only β3 subunit expression. CONCLUSIONS: In light of the critical role played by eicosanoids in endometrial differentiation, we suggest that PGE 2 is also involved in local paracrine regulation of integrin expression.