Premium
Granulocyte colony stimulation factor (G‐CSF) suppresses interleukin (IL)‐12 and/or IL‐2 induced interferon (IFN)‐γproduction and cytotoxicity of decidual mononuclear cells
Author(s) -
Sugita Kenji,
Hayakawa Satoshi,
KarasakiSuzuki Miki,
Hagiwara Hiroshi,
Chishima Fumihisa,
Aleemuzaman Sheikh,
Li Jonga,
Nishinarita Susumu,
Yamamoto Tatsuo
Publication year - 2003
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2003.00024.x
Subject(s) - biology , peripheral blood mononuclear cell , decidua , interleukin , interleukin 15 , interferon gamma , immunology , trophoblast , cytokine , endocrinology , microbiology and biotechnology , medicine , placenta , pregnancy , fetus , in vitro , biochemistry , genetics
Problem: The placenta is one of the few non‐hematopoietic tissues to express granulocyte colony stimulation factor (G‐CSF). Placental G‐CSF production is considered to be one of the major causes of granulocytosis during pregnancy although its physiological role in pregnancy has not yet been examined. Method of study: The effects of G‐CSF on interleukin (IL)‐2 and/or IL‐12 induced interferon (IFN)‐γ production of magnetic cell sorting (MACS) sorted decidual lymphocytes was examined by enzyme‐linked immunosorbent spot‐forming cell assay (ELISPOT). The effect of G‐CSF on cytotoxicity of decidual lymphocytes against the choriocarcinoma cell line JEG‐3 was examined by lactate dehydrogenase (LDH) release assay. Results: As previously reported by us, IL‐2 and/or IL‐12 activated decidual mononuclear cells were capable of killing choriocarcinoma cells. We observed that G‐CSF abolished IFN‐γ production and cytotoxicity of decidual mononuclear cells and MACS sorted CD56 + cells. Conclusions: In addition to its well‐known trophic effects on hematopoiesis, our results suggest about new roles of G‐CSF in reproductive immunology.