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Intracellular Expression of CD69 in Endometrial and Peripheral T cells Represents a Useful Marker in Women with Recurrent Miscarriage: Modulation After Allogeneic Leukocyte Immunotherapy
Author(s) -
Ramhorst R.,
García V.,
Agriello E.,
Corigliano A.,
Etchepareborda E.,
Irigoyen M.,
Pasanante Gómez,
Fainboim L.
Publication year - 2003
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2003.00021.x
Subject(s) - cd69 , flow cytometry , recurrent miscarriage , endometrium , intracellular , biology , andrology , immunology , endocrinology , medicine , pregnancy , t cell , immune system , miscarriage , il 2 receptor , microbiology and biotechnology , genetics
PROBLEM: To characterize in fertile women and women with recurrent spontaneous abortions (RSA) the expression and functional status of T cells expressing the CD69 molecule. METHOD OF STUDY: We analyzed by flow cytometry in peripheral blood and endometrium from fertile and RSA women, the surface and cytoplasmic expression of CD69 on gated T cells. In addition, we investigated by three‐color flow cytometry the expression of cytokines, and subsets of memory T cells. RESULTS: In T cells, CD69 was restricted to the intracellular compartment with a higher frequency in RSA than in fertile women (68.2 ± 12% versus 23.7 ± 22%, P < 0.001, and 20 ± 9.5% versus 2.1 ± 3.8%, P < 0.005, in endometrium and peripheral blood, respectively). In contrast, the number of interferon‐γ + (IFN‐γ + ) secreting cells was higher (16 ± 5% versus 6 ± 1%) in fertile women. All 11 RSA women alloimmunized with parental leukocytes reached values of CD3 + CD69 + cells similar to those observed in fertile women. CONCLUSIONS: CD69 might represent a useful marker in the diagnosis and the follow up of RSA patients.