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In Vitro Modulation of Protective Antibody Responses by Estrogen, Progesterone and Interleukin‐6[Note 1. Presented at the ASRI XXIst Annual Meeting, Chicago, June ...]
Author(s) -
Canellada Andrea,
Blois Sandra,
Gentile Teresa,
Margni Idehu Ricardo A.
Publication year - 2002
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2002.01141.x
Subject(s) - antibody , estrogen , microbiology and biotechnology , biology , western blot , estrogen receptor , interleukin 2 , immune system , interleukin , endocrinology , in vitro , cell growth , medicine , chemistry , cytokine , immunology , biochemistry , cancer , breast cancer , gene , genetics
Canellada A, Blois S, Gentile T, Margni Idehu RA. In vitro modulation of protective antibody responses by estrogen, progesterone and interleukin‐6. AJRI 2002; 48:334–343 © Blackwell Munksgaard, 2002 PROBLEM: We have previously demonstrated that the addition of placental interleukin‐6 (IL‐6) to murine hybridomas increased asymmetric antibody synthesis. Here we analyze whether progesterone (Pg) and estrogen (E 2 ) affect asymmetric antibody synthesis by modulating IL‐6 production in hybridoma cells. METHOD OF STUDY: Hybridoma 112D5 B cells were cultured with E 2 , Pg or recombinant IL‐6. Cell proliferation was assessed by 3 H‐thymidine incorporation, and asymmetric antibodies were measured in culture supernatants by Con A fixation and enzyme‐linked immunusorbant assay (ELISA). E 2 and Pg‐receptors (ER and PR) were evaluated in whole cell extracts by Western blot. IL‐6 was measured in culture supernatants by ELISA. RESULTS: 112D5 expressed both PR and ER, which were differentially regulated. At 48 hr, Pg and E 2 slightly decreased cell proliferation whereas IL‐6 did not. As well as IL‐6, 10 –10 M Pg but not E 2 induced asymmetric antibody production. Interestingly, Pg at 10 –6 M decreased asymmetric antibody synthesis by hybridoma cells. Finally, mainly Pg but also E 2 increased IL‐6 synthesis, although IL‐6 levels did not correlate with asymmetric antibodies synthesized in the presence of E 2 or Pg. CONCLUSIONS: In cells expressing both ER and PR, we could demonstrate that steroids participate in humoral immune responses by modulating asymmetric antibody synthesis. IL‐6 proved to be only partially involved. Other possible mechanisms involved in the effect of Pg on blocking antibody responses and their contribution to a successful pregnancy are discussed in the paper.