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Absence of HLA‐G Expression in Macrophages of Human Decidua[Note 1. Presented at the VIII International Congress of Reproductive Immunology, ...]
Author(s) -
SEDLMAYR PETER,
MORALES PEDRO,
TRUMMER SILVIA,
WASCHER KARIN,
AZZOLA DAGMAR,
BLASCHITZ ASTRID,
HAMMER ASTRID,
WALCHER WOLFGANG,
WINTERSTEIGER REINHOLD,
DOHR GOTTFRIED
Publication year - 2002
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2002.01116.x
Subject(s) - decidua , biology , flow cytometry , hla g , immunohistochemistry , macrophage , immunology , human leukocyte antigen , immune system , microbiology and biotechnology , antigen , placenta , fetus , in vitro , pregnancy , biochemistry , genetics
PROBLEM: Macrophages – together with natural killer (NK) cells – constitute the majority of bone marrow derived infiltrating cells in the decidua. As interferon‐γ (IFN‐γ), a cytokine produced by NK cells, has been reported to induce expression of human leukocyte antigen (HLA‐G) in monocytic cells, suggesting expression of HLA‐G on decidua macrophages potentially stimulated by IFN‐γ, the question arises whether decidua macrophages in normal pregnancy express HLA‐G. METHOD OF STUDY: The study was based on immunohistochemistry and flow cytometry. In order to exclude that potentially elusive soluble HLA‐G was not detected by immunohistochemistry, we performed in addition RT–PCR of flow‐sorted decidua macrophages. RESULTS: Our findings indicate that HLA‐G is not present on macrophages of first trimester or term decidua in either membrane‐bound or soluble form. Transcripts for soluble HLA‐G1 and ‐G2 were not detected. CONCLUSIONS: We exclude a role of HLA‐G on the surface of decidua macrophages or of soluble HLA‐G1 or ‐G2 as a secretory product of decidua macrophages with regard to interaction with HLA‐G receptors present in or outside the decidua.