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Transforming Growth Factors β1, β2 and β3 and their Receptors are Differentially Expressed in Human Peritoneal Fibroblasts in Response to Hypoxia
Author(s) -
Saed Ghassan M.,
Collins Karen L.,
Diamond Michael P.
Publication year - 2002
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2002.01090.x
Subject(s) - transforming growth factor , receptor , medicine , endocrinology , messenger rna , hypoxia (environmental) , biology , transforming growth factor beta , chemistry , gene , biochemistry , oxygen , organic chemistry
Saed GM, Collins KL, Diamond MP. Transforming growth factors β1, β2, and β3 and their receptors are differentially expressed in human peritoneal fibroblasts in response to hypoxia. AJRI 2002; 48: 387–393 © Blackwell Munksgaard, 2002 PROBLEM: Little is known about the role of peritoneal fibroblasts in adhesion formation. This study determines the effect of hypoxia and transforming growth factor (TGF)‐β1 treatment on the expression of TGF‐β1–3 and TGF‐βI and βII receptors in human peritoneal fibroblasts (HPF). TGF‐β isoforms and their receptors have been implicated as mediators of the healing process and adhesion development. METHODS: HPF were cultured under normal and hypoxic condition, and treated with and without (1 ng/mL) TGF‐β1 for 24 hr. Total RNA from each group was subjected to multiplex reverse transcriptase‐polymerase chain reaction (RT/PCR) to quantitate TGF‐β1–3 and TGF‐βI and βII receptors messenger RNA (mRNA) levels. RESULTS: Hypoxia resulted in a significant increase in TGF‐β1 (26%; P < 0.05), TGF‐βIR (34%; P < 0.05) and TGF‐βIIR (29%; P < 0.05) mRNA levels, with no effect on TGF‐β2 or β3. TGF‐β1 treatment resulted in a significant increase in TGF‐β1 (35%; P < 0.05), but a decrease in TGF‐β2 (22%; P < 0.05) and no effect on TGF‐β3, TGF‐βIR or TGF‐βΙIR. Combined treatment of hypoxia and TGF‐β1 caused a significant increase in TGF‐β1 (37%; P < 0.05), TGF‐β2 (12%; P < 0.05), TGF‐βIR (32%; P < 0.05) and TGF‐βIIR (34%; P < 0.05). There is no significant change in the mRNA levels of TGF‐β3 in any of the treatments. CONCLUSION: Hypoxia and TGF‐β1 treatments of cultured HPF modulate the expression of TGF‐β1, β2 and β3 and their receptors βIR and βIIR by increasing the ratio of TGF‐β1 and β2 to β3, thus favoring the development of peritoneal adhesion.