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Differential Effects of IL‐11 on Rat Blastocysts and Decidua during the Peri‐Implantation Period
Author(s) -
CALUWAERTS S.,
PIJNENBORG R.,
LUYTEN C.,
KEITH J.C.,
VAN ASSCHE F.A.
Publication year - 2002
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2002.01070.x
Subject(s) - decidualization , blastocyst , decidua , uterus , endocrinology , hatching , medicine , andrology , peri , embryo , biology , embryogenesis , pregnancy , placenta , fetus , microbiology and biotechnology , zoology , genetics
PROBLEM: To study effects of interleukin‐11 (IL‐11) on blastocyst development and decidualization. METHOD OF STUDY: Rats, injected with buffer (C) or IL‐11 [1 mg/kg/day=high dose (HD), 60 μg/kg/week=low dose (LD)‐1, 30 μg/kg twice a week=low dose (LD)‐2] were made pregnant or pseudopregnant to obtain blastocysts or deciduomata. RESULTS: As compared with C, more LD‐2 blastocysts hatched in culture, while hatching and attachment of HD blastocysts was decreased. Blastocysts from untreated rats in IL‐11 supplemented medium (4 ng/mL) demonstrated increased hatching and attachment. The weight of the decidualized uterus in HD and LD‐2 pseudopregnant rats was reduced as compared with C and LD‐1. On deciduomata sections from IL‐11 treated rats, the area inside the uterine muscle layer was reduced, and mitotic over pycnotic indices were increased in the anti‐mesometrial area and decreased in the mesometrial area. CONCLUSIONS: Low doses of IL‐11 improve hatching and attachment of blastocysts, but both high and low doses impair decidualization.