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Enhancement of In Vitro hsp72 Expression by Placental IL‐6
Author(s) -
MIRANDA S.,
GENTILE T.,
MARGNI R.
Publication year - 2001
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1034/j.1600-0897.2001.d01-24.x
Subject(s) - in vitro , biology , antibody , placenta , hsp70 , microbiology and biotechnology , placental lactogen , antigen , cell culture , heat shock protein , immunology , gene , biochemistry , fetus , pregnancy , genetics
PROBLEM: To give an approach in order to elucidate the mechanism by which placental IL‐6 induce modifications in the glycosylation status of immunoglobulins, in the present work, we investigate a putative relationship between a stimulus by placental IL‐6 and expression of cytoplasmic “hsp70 family proteins” in an in vitro model.
METHODS OF STUDY: Supernatants of cultures of placentae obtained from primiparous and multiparous AKR/J×AKR/J and AKR/J×BALB/c mouse crossbreedings were added to mouse IgG1 hybridoma cultures which produced symmetric and asymmetric anti‐dinitrophenol (anti‐DNP) antibodies. Analyses of the expression of inducible hsp72/constitutive hsp73 in cellular lysates obtained from hybridomas cultured, in the presence of rmIL‐6 or crude murine placental culture supernatants, followed by neutralization assays with anti IL‐6, were performed. In addition, the level of IL‐6 present in the employed placental culture supernatants was determined and compared with the placental hsp70‐inducing effect.
RESULTS: These experiments showed that mouse placentae were able to release IL‐6 in vitro . In addition, mouse placental supernatants (PS) containing over 1,000 pg/mL of IL‐6 enhanced the expression of the inducible isoform hsp72 in the employed hybridomas. This effect was abolished when the hsp70‐inducing PS were previously incubated with anti‐mIL6 antibody.
CONCLUSIONS: These observations indicate that mouse placentae produce different titers of IL‐6 and suggest that IL‐6 appears to be the unique mouse placental factor able to induce in vitro hsp72 synthesis. A relationship with the increased synthesis of anti‐paternal antigen asymmetric antibodies, previously observed during pregnancy, is discussed.