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Immunocytochemical study of activin type IB receptor (XALK4) in Xenopus oocytes
Author(s) -
Fukui Akimasa,
Komazaki Shinji,
Miyoshi Osamu,
Asashima Makoto
Publication year - 2003
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1034/j.1600-0854.2004.00680.x
Subject(s) - xenopus , activin receptor , immunocytochemistry , acvr2b , microbiology and biotechnology , biology , zona pellucida , immunoelectron microscopy , antiserum , endoplasmic reticulum , receptor , medicine , endocrinology , oocyte , signal transduction , antibody , biochemistry , tgf beta signaling pathway , transforming growth factor , immunology , embryo , gene
Studies have shown that the activin type IB receptor is specific for activin/nodal signaling. Activin is produced by follicle cells in the ovary, and is incorporated into the oocytes. Antisera against three peptides were prepared, encompassing the extracellular, intracellular and serine/threonine kinase domains of the Xenopus type IB activin receptor (XALK4). Immunocytochemistry was done using these antisera to investigate the distribution of XALK4 in the Xenopus ovary. All three antisera stained the mitochondrial cloud of Xenopus previtellogenic oocytes. Purified antibody against the intracellular domain also recognized the mitochondrial cloud. Immunoelectron microscopy localized XALK4 on the endoplasmic reticulum of the mitochondrial cloud, although not on mitochondria.