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A Kinetic Proof‐reading Mechanism for Protein Sorting
Author(s) -
Weiss Matthias,
Nilsson Tommy
Publication year - 2003
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2003.40202.x
Subject(s) - copi , adp ribosylation factor , gtpase , biology , golgi apparatus , microbiology and biotechnology , gtp' , cytosol , gtp binding protein regulators , gtpase activating protein , sorting , biophysics , biochemistry , enzyme , g protein , secretory pathway , signal transduction , endoplasmic reticulum , computer science , programming language
Resident proteins of the exocytic pathway are maintained at various levels through coatomer protein I (COPI)‐mediated recycling. Sorting of cargo by COPI requires GTP hydrolysis by ADP‐ribosylation factor 1 (ARF‐1). This small GTPase recruits coatomer onto Golgi membranes and upon hydrolysis, is thought to release coatomer back into the cytosol. This step requires the activating protein, ARFGAP1. By coupling sorting to a cargo‐induced sequestering of ARFGAP1, we have formulated a kinetic proof‐reading model that explains how a GTP hydrolysis‐driven coat release can yield an active sorting event. The sorting scheme predicts a dependency on the amount of ARFGAP1 and explains the recent experimental findings that ARF‐1 and COPI detach with different time constants from the Golgi membrane in vivo .