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The Overexpression of GMAP‐210 Blocks Anterograde and Retrograde Transport Between the ER and the Golgi Apparatus
Author(s) -
PernetGallay Karin,
Antony Claude,
Johannes Ludger,
Bornens Michel,
Goud Bruno,
Rios Rosa M.
Publication year - 2002
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2002.31107.x
Subject(s) - golgi apparatus , endoplasmic reticulum , biology , microbiology and biotechnology , copi , microtubule , protein subunit , secretory pathway , copii , axoplasmic transport , transport protein , biochemistry , gene
Golgi Microtubule‐Associated Protein (GMAP)‐210 is a peripheral coiled‐coil protein associated with the cis ‐Golgi network that interacts with microtubule minus ends. GMAP‐210 overexpression has previously been shown to perturb the microtubule network and to induce a dramatic enlargement and fragmentation of the Golgi apparatus (Infante C, Ramos‐Morales F, Fedriani C, Bornens M, Rios RM. J Cell Biol 1999; 145: 83–98). We now report that overexpressing GMAP‐210 blocks the anterograde transport of both a soluble form of alkaline phosphatase and the hemagglutinin protein of influenza virus, an integral membrane protein, between the endoplasmic reticulum and the cis /medial (mannosidase II‐positive) Golgi compartment. Retrograde transport of the Shiga toxin B‐subunit is also blocked between the Golgi apparatus and the endoplasmic reticulum. As a consequence, the B‐subunit accumulates in compartments positive for GMAP‐210. Ultrastructural analysis revealed that, under these conditions, the Golgi complex is totally disassembled and Golgi proteins as well as proteins of the intermediate compartment are found in vesicle clusters distributed throughout the cell. The role of GMAP‐210 on membrane processes at the interface between the endoplasmic reticulum and the Golgi apparatus is discussed in the light of the property of this protein to bind CGN membranes and microtubules.