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Endosomal Sorting of Amyloid Precursor Protein‐P‐Selectin Chimeras Influences Secretase Processing
Author(s) -
Daugherty Brandy L.,
Green Samuel A.
Publication year - 2001
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2001.21206.x
Subject(s) - biology , endosome , amyloid precursor protein , microbiology and biotechnology , amyloid precursor protein secretase , amyloid (mycology) , retromer , alzheimer's disease , medicine , disease , intracellular , botany
Amyloid β protein, the major component of the senile plaques in Alzheimer's disease, is generated by secretory and endocytic processing of amyloid precursor protein. Internalized amyloid precursor protein either recycles to the plasma membrane, where α‐secretase resides, or moves to acidic compartment(s) for β‐secretase exposure. While the trans‐Golgi network contains β‐secretase activity, recent examination of the subcellular distribution of this proteinase, called BACE, has led to the suggestion that β‐secretase activity might also reside at the plasma membrane and in endosomes. To examine the role of endocytic compartments in β‐secretase processing of amyloid precursor protein, the wild‐type and endosomal sorting mutant P‐selectin cytoplasmic domains were used to control movement of amyloid precursor protein through endosomes. Amyloid precursor protein/P‐selectin, which is sorted from early to late endosomes, undergoes significantly less α‐secretase cleavage, and more β‐secretase cleavage, than amyloid precursor protein/P‐selectin768A, a mutant that recycles more efficiently to the cell surface. Our results demonstrate that endosomal sorting influences relative exposure of the amyloid precursor protein/P‐selectin chimeras to α‐ and β‐secretase activities, and suggest that, because delivery to late endocytic compartments favors β‐secretase processing of amyloid precursor protein, there is likely limited β‐secretase activity in early endosomes or at the cell surface. We propose that the trans‐Golgi network may be involved in both secretory and endocytic generation of amyloid β protein.

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