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Strategies for Prokaryotic Expression of Eukaryotic Membrane Proteins
Author(s) -
Laage Rico,
Langosch Dieter
Publication year - 2001
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2001.020204.x
Subject(s) - biology , microbiology and biotechnology , membrane protein , expression (computer science) , eukaryotic cell , computational biology , membrane , genetics , cell , computer science , programming language
High‐level heterologous expression of integral membrane proteins at full‐length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.