Phagocytosis and Macropinocytosis in Dictyostelium : Phosphoinositide‐Based Processes, Biochemically Distinct

Phagocytosis and macropinocytosis are actin‐dependent clathrin‐independent processes primarily performed by cells like neutrophils and macrophages that result in the internalization of particles or the formation of fluid‐filled macropinosomes, respectively. Phagocytosis consists of a number of stages, including attachment of particles to cell surface receptors, engulfment of the particle dependent on actin polymerization and membrane exocytosis, and formation of phago‐lysosomes. In contrast, the molecular steps regulating macropinocytosis are only just now being deciphered. Much remains to be learned concerning the signaling pathways that regulate these processes. Dictyostelium is a genetically and biochemically tractable professional phagocyte that has proven to be a powerful system with which to determine the nature of the molecular steps involved in regulating these internalization processes. This review summarizes what is currently understood concerning the molecular mechanisms governing phagocytosis and macropinocytosis in Dictyostelium and describes recent data concerning the common and distinct pathways that regulate these processes.