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Targeting of HIV‐1 Nef to the Centrosome: Implications for Antigen Processing
Author(s) -
Lacaille Vashti G.,
Androlewicz Matthew J.
Publication year - 2000
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2000.011107.x
Subject(s) - biology , centrosome , microbiology and biotechnology , human immunodeficiency virus (hiv) , antigen , antigen processing , virology , computational biology , immunology , genetics , major histocompatibility complex , cancer , cell cycle , mhc class i
To gain a better understanding of the intracellular sites of antigen processing we have looked at the localization of human immunodeficiency virus (HIV)‐1 Nef protein by confocal microscopic and biochemical means. We found that ubiquitin (Ub)‐Nef fusion proteins were localized to the centrosome in transfected COS‐7 cells, and that the colocalization was inhibited by the microtubule‐disrupting agent, nocodazole. Interestingly, we found that Ub‐Nef trafficking to the centrosome was not dependent upon the metabolic stability of Ub‐Nef nor on the inhibition of proteasome activity. We also analyzed the MHC class I antigen processing of a reporter epitope linked to the Ub‐Nef fusion proteins and found that Ub‐Nef was processed in COS‐7 cells. In addition, we show that this processing was inhibited by nocodazole. We suggest that the centrosome may serve as a site of antigen processing in vivo .