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The Use of Yeast Two‐Hybrid Screens in Studies of Protein:Protein Interactions Involved in Trafficking
Author(s) -
Stephens David J.,
Banting George
Publication year - 2000
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2000.011003.x
Subject(s) - biology , yeast , microbiology and biotechnology , protein–protein interaction , transport protein , two hybrid screening , computational biology , genetics
The yeast two‐hybrid system has provided a convenient means to both screen for proteins that interact with a protein of interest and to characterise the known interaction between two proteins. Several groups with an interest in the molecular mechanisms that underlie discrete steps along trafficking pathways have exploited the yeast two‐hybrid system. Here, we provide a brief background to the technology, attempt to point out some of the pitfalls and benefits of the different systems that can be employed, and mention some of the areas (within the trafficking field) where yeast two‐hybrid interaction assays have been particularly informative.