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A Screen of Random Sequences for those that Alter the Trafficking of the Influenza Virus Hemagglutinin In Vivo
Author(s) -
Lewis Cheryl M.,
Latham Katrina,
Roth Michael G.
Publication year - 2000
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2000.010310.x
Subject(s) - internalization , biology , hemagglutinin (influenza) , cytoplasm , virology , virus , microbiology and biotechnology , endocytosis , recombinant dna , genetics , gene , receptor
In order to determine if the sequence patterns known to specify internalization represent the majority of possible internalization signals, we identified random sequences capable of causing a reporter protein to be internalized at least several‐fold faster than the rate of non‐selective internalization of membrane by clathrin‐coated pits. A library of influenza hemagglutinin (HA) proteins, bearing short random sequences in place of the wild‐type cytoplasmic domain, was prepared in recombinant SV40 virus. The library was expressed and screened for HAs that could internalize anti‐HA antibody from the medium. The cytoplasmic sequences of the selected proteins were determined. From a small sample of sequences, we detected several that did not resemble those previously identified. The known internalization signals must represent only a subset of the sequences that can serve as internalization signals.