Open AccessElectron paramagnetic resonance study utilizing stripping method on normal human stratum corneum
Author(s) -
Mizushima Junichi,
Kawasaki Yoshiaki,
Kitano Takeshi,
Sakamoto Kazutami,
Kawashima Makoto,
Cooke Roger,
Maibach Howard I.
Publication year - 2000
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1034/j.1600-0846.2000.006003108.x
Subject(s) - stratum corneum , electron paramagnetic resonance , spectral line , stripping (fiber) , chemistry , analytical chemistry (journal) , membrane , nitroxide mediated radical polymerization , nuclear magnetic resonance , materials science , chromatography , polymer , composite material , medicine , biochemistry , physics , radical polymerization , organic chemistry , pathology , astronomy , copolymer
Background/aim: Electron paramagnetic resonance (EPR) spectra of nitroxide spin probes are useful for studying biological membranes, and chemical‐membrane interaction. The objective of this study was to assess the fluidity of stripped stratum corneum (SC) of normal human skin with EPR. Methods: We quantified the fluidity of stripped SC (S‐SC) by a single stripping with cyanoacrylate onto quartz glass, cover glass or transparent film. Spectra were compared with those of the SC of cadaver skin (C‐SC). Results: All S‐SC spectra were similar to those of C‐SC. There were no statistical differences in the order parameters S between C‐SC and those on quartz cell and cover glass, but those of transparent film were significantly higher. Spectra after a month storage at room temperature at 5°C and at −20°C were unchanged. The order parameter S had decreased after a 3‐month storage in all conditions. Conclusion: This method is useful for the evaluation of the fluidity measurement utilizing EPR spectra of S‐SC.