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Melatonin related compounds inhibit lipid peroxidation during copper or free radical‐induced LDL oxidation
Author(s) -
BonnefontRousselot Dominique,
Chevé Gwénaël,
Gozzo Andrea,
Tailleux Anne,
Guilloz Virginie,
Caisey Stéphanie,
Teissier Elisabeth,
Fruchart JeanCharles,
Delattre Jacques,
Jore Daniel,
Lesieur Daniel,
Duriez Patrick,
GardèsAlbert Monique
Publication year - 2002
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1034/j.1600-079x.2002.02911.x
Subject(s) - melatonin , chemistry , lipid peroxidation , tbars , radical , antioxidant , free radical scavenger , lipid oxidation , superoxide , biochemistry , endocrinology , enzyme , biology
This study was designed to evaluate the protective effect of two melatonin related compounds towards low density lipoproteins (LDL) oxidation initiated in vitro either by defined free radicals [i.e. superoxide anion (O 2 · – ) and ethanol‐derived peroxyl radicals (RO 2 · )] produced by gamma radiolysis or by copper ions. The compounds studied were N‐[2‐(5‐methoxy‐1H‐indol‐3‐yl)ethyl]‐3,5‐di‐tert‐butyl‐4‐hydroxybenzamide (DTBHB) and (R,S)‐1‐(3‐methoxyphenyl)‐2‐propyl‐1,2,3,4‐tetrahydro‐β‐carboline (GWC20) which is a pinoline derivative. Their effects were compared with those of melatonin at the same concentration (100 μ mol/L). None of the three tested compounds protected endogenous LDL α‐tocopherol from oxidation by RO 2 · /O 2 · – free radicals. By contrast, they all protected β‐carotene from the attack of these free radicals with GWC20 being the strongest protector. Moreover, melatonin and DTBHB partially inhibited the formation of products derived from lipid peroxidation (conjugated dienes and thiobarbituric acid‐reactive substances or TBARS) while GWC20 totally abolished this production. As previously shown, melatonin (at the concentration used) inhibited copper‐induced LDL oxidation by increasing 1.60‐fold the lag phase duration of conjugated diene formation over the 8 hr of the experimental procedure, however, DTBHB and GWC20 were much more effective, because they totally prevented the initiation of the propagation phase of LDL oxidation. It would be interesting to test in vivo if DTBHB and GWC20 which exhibit a strong capacity to inhibit in vitro LDL oxidation would reduce or not atherosclerosis in animals susceptible to this pathology.