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Identification and sequencing of pineal‐specific enhancing region in the mouse tryptophan hydroxylase promoter
Author(s) -
Yim SungVin,
Huh SungOh,
Kim JongWoo,
Kim MiJa,
Park SeungJoon,
Jung JeeChang,
Chung JooHo
Publication year - 2000
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1034/j.1600-079x.2001.280206.x
Subject(s) - tryptophan hydroxylase , biology , gene , pineal gland , microbiology and biotechnology , gene expression , regulatory sequence , promoter , transgene , sequence analysis , genetics , melatonin , endocrinology , serotonin , receptor , serotonergic
Pineal‐specific expression of the tryptophan hydroxylase (TPH) gene has been demonstrated by a number of studies. However, little is known about the regulatory mechanism for pineal‐specific expression of the TPH gene. To identify the cis ‐acting region responsible for pineal‐specific expression of the TPH gene, we investigated a 6.1‐kb 5′‐flanking region of the mouse TPH gene using an immortalized pineal cell line (PGT‐β) derived from transgenic mice. By deletion analysis, it was demonstrated that the pineal‐specific enhancing region resides approximately between −6.1 and −4.7 kb upstream from the transcription initiation site of the mouse TPH gene. Additionally, nucleotide sequence analysis of this region showed that the (AC/TG) 22 repetitive sequence is located approximately −5.78 kb upstream of the mouse TPH gene, and several known tissue‐specific cis ‐acting elements, such as Pit‐1 and the pituitary specific element (PSE), have also been identified in the region. We believe that the analysis of the sequence and several cis ‐acting elements in the pineal‐specific enhancing region of the mouse TPH promoter would enhance our understanding of the precise mechanism of pineal‐specific expression.