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The effect of melatonin on in vitro fertilization and embryo development in mice
Author(s) -
Ishizuka Bunpei,
Kuribayashi Yasushi,
Murai Kunihiko,
Amemiya Akira,
Itoh Masanori T.
Publication year - 2000
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1034/j.1600-079x.2000.280107.x
Subject(s) - melatonin , human fertilization , embryo , andrology , embryogenesis , in vitro fertilisation , biology , insemination , in vitro , fertilisation , endocrinology , medicine , sperm , anatomy , reproductive technology , microbiology and biotechnology , biochemistry
To examine the effect of melatonin on in vitro fertilization and embryonic development, mouse embryos after insemination in vitro were cultured in a physiological medium with or without melatonin. Melatonin increased the fertilization rate significantly at a concentration between 10 −6 and 10 −4 M (27.6 vs. 43.9 or 40.4%, P <0.01). Furthermore, a significant increase in the rate of embryos reaching the four‐cell stage (16.0 vs. 26.7%, P <0.01), the eight‐cell stage (12.1 vs. 25.8 or 23.5%, P <0.01), and blastulation (8.9 vs. 23.5 or 17.5%, P <0.01) was observed when the embryos were cultured in a medium containing 10 −8 or 10 −6 M melatonin. These results demonstrate that melatonin supports fertilization and early embryo development after in vitro fertilization.