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New source for evaluating PDT drugs in the laboratory
Author(s) -
Sayre R.M.,
Dowdy J.C.,
Gart M.
Publication year - 2002
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1034/j.1600-0781.2002.180208_18.x
Subject(s) - light source , materials science , interference (communication) , excitation , optics , halogen lamp , optoelectronics , biomedical engineering , computer science , physics , engineering , telecommunications , electrical engineering , channel (broadcasting)
Laboratory comparison of different PDT drugs can pose a number of problems because of sources with different exposure geometries and excitation wavebands. This can be overcome by utilizing a single source consisting of a quartz halogen source filtered with appropriate narrow band pass, interference type filters. The source is modified to provide eight simultaneous exposures to samples in 96 well tissue culture plates. This enables a substantial number of exposures to be performed for comparative effectiveness studies. By simply changing optical band pass filters different drugs can be tested in the same plates or subsequent plates. By irradiating each plate from the bottom, all wells can be comparably exposed irrespective of media volume.