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Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells
Author(s) -
Lee David M.,
Gasparro Francis P.,
Wang Xiu Jie,
Kopec Chris,
DeLeo Kier,
Sumpio Bauer E.
Publication year - 2002
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1034/j.1600-0781.2002.02770.x
Subject(s) - cytostasis , vascular smooth muscle , cell growth , restenosis , smooth muscle , chemistry , phototoxicity , in vitro , andrology , endothelium , microbiology and biotechnology , biophysics , medicine , biology , surgery , biochemistry , cytotoxicity , stent
Background: The long‐term efficacy of percutaneous transluminal coronary angioplasty is limited by the restenosis which occurs in approximately 40% of patients, usually within 6 months of the procedure. Purpose: The present study was designed to evaluate the effects of 8‐methoxypsoralen (8‐MOP) activated with visible light on the properties of bovine aortic smooth muscle cells (SMC) and endothelial cells (EC) in vitro . Methods: Cells were seeded in polystyrene wells, allowed to attach over a 24‐h period, incubated with 1, 20, or 50 µg/ml 8‐MOP and then exposed to 12 J/cm 2 visible light (447 nm). Cell counts were performed for up 14 days ( n = 4–6 wells per time point), and each experiment was performed in triplicate. Cellular migration, morphology, and size were also analyzed. Results: The lowest 8‐MOP dose (1 µg/ml) had no significant effect on SMC proliferation, while the highest dose (50 µg/ml) induced cytostasis. An intermediate dose of 8‐MOP (20 µg/ml) produced a transient and reversible inhibition of proliferation. There was no significant effect on proliferation of EC at lowest dose of 8‐MOP (1 µg/ml). However, in contrast to the SMC experiments, a transient and reversible inhibition of EC proliferation was seen at both 20 and 50 µg/ml 8‐MOP. Conclusions: These experiments demonstrate that while 8‐MOP photoactivated with 447 nm visible light can reversibly inhibit the proliferation of both SMC and EC in a dose‐dependent fashion, SMC are more sensitive to the treatment than EC.