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UVB‐induced GM‐CSF production is suppressed by dexamethasone in HaCaT Cells
Author(s) -
Kim Dong Seok,
Kim Hye Jin,
Choi Kyoung Hee,
Chung JinHo,
Kim Kyu Han,
Park Kyoung Chan
Publication year - 2001
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1034/j.1600-0781.2001.170303.x
Subject(s) - hacat , dexamethasone , granulocyte macrophage colony stimulating factor , secretion , basal (medicine) , endocrinology , chemistry , glucocorticoid , medicine , in vitro , biology , cytokine , biochemistry , insulin
Background: Epidermal keratinocytes are important sources of a wide variety of cytokines that include the Granulocyte‐macrophage Colony Stimulating Factor (GM‐CSF). Glucocorticoids have been shown to inhibit the production of several cytokines. However, their effect on GM‐CSF synthesis by keratinocytes is still unknown. Methods: The effects of glucocorticoid on GM‐CSF production by keratinocytes were evaluated using ELISA and RT‐PCR analysis. Results: GM‐CSF secretion by HaCaT cells increased with increasing UVB exposure. Dexamethasone suppressed basal release of GM‐CSF. In addition, it strongly inhibited both the UVB‐mediated augmentation of GM‐CSF protein production and mRNA expression. Lincomycin enhanced slightly the inhibitory effect of dexamethasone on GM‐CSF synthesis, while lincomycin itself had no effect on GM‐CSF secretion. Conclusion: Results showed that dexamethasone suppressed basal release and UVB‐induced production of GM‐CSF by keratinocytes.