Open AccessComparison of the Embryotoxic Effects of Saporin, Agrostin (Type 1 Ribosome‐Inactivating Proteins) and Ricin (a Type 2 Ribosome‐Inactivating Protein
Author(s) -
Chan WoodYee,
Ng TziBun
Publication year - 2001
Publication title -
pharmacology & toxicology
Language(s) - English
Resource type - Journals
eISSN - 1600-0773
pISSN - 0901-9928
DOI - 10.1034/j.1600-0773.2001.880603.x
Subject(s) - ricin , saporin , ribosome inactivating protein , protein biosynthesis , endocrinology , biology , ribosome , medicine , chemistry , microbiology and biotechnology , biochemistry , rna , immunotoxin , cytotoxicity , toxin , gene , in vitro
The effects of two type I ribosome‐inactivating proteins, saporin and agrostin, and the type 2 ribosome‐inactivating protein ricin and its constituent A and B chains, on the development of cultured mouse embryos, were investigated. Saporin and agrostin had similar embryotoxicity which was approximately 10 5 times weaker than that of ricin and its A and B chains, and the embryotoxicity of saporin and agrostin increased gradually with the dosage. In contrast, there was an abrupt rise in embryotoxictiy of ricin‐A chain and B chain as the dose was raised from 10 to 20 ng ml −1 . Saporin and agrostin did not affect the heart‐beat and otic placode, but at 10 ng ml −1 ricin and 40 ng ml −1 ricin‐A chain and B chain, all of the treated embryos exhibited abnormalities in heart‐beat, yolk sac circulation, body axis, optic placode, otic placode, forelimb buds, branchial apparatus and cranial neural tube.